Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Let-7c-5p/IGF2BP1 axis in Oral Squamous Cell Carcinoma represses progressions in vitro

Yan Wang, Hui Ren, Qing Wang, Shuang Li, Yanhua Lu, Wei Masu, Juan Zhang, Tao Wang

The Second Central Hospital Dental Baoding City, Hebei Province, 072750, China;

For correspondence:- Tao Wang Email: vfj89895@126.com Tel:+8617764436922

Accepted: 10 August 2021 Published: 30 December 2021

Citation: Wang Y, Ren H, Wang Q, Li S, Lu Y, Masu W, et al. Let-7c-5p/IGF2BP1 axis in Oral Squamous Cell Carcinoma represses progressions in vitro. Trop J Pharm Res 2021; 20(12):2511-2518 doi: 10.4314/tjpr.v20i12.8

© 2021 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: Oral squamous cell carcinoma (OSCC), a malignant tumor with high mortality, occurs in the head and neck. Let-7c-5p is involved in progression of many kinds of cancers. The purpose of this study was to determine the biological function of let-7c-5p in OSC.

Methods: RT-qPCR was performed to determine expressions of let-7c-5p and western blot measured protein expressions of IGF2BP1, E-cadherin, Vimentin and N-cadherin. Furthermore, dual-luciferase reporter test was used to evaluate the binding between IGF2BP1 and let-7c-5p. Moreover, MTT assay was employed to evaluate the cell viability while Transwell and Scratch assays were used to confirm cell metastasis. The cell apoptosis was validated using flow cytometry.

Results: Downregulation of let-7c-5p was recognized in OSCC cell lines. Let-7c-5p directly targeted and negatively modulated IGF2BP1. Other assays showed that let-7c-5p up-regulation had significant inhibitory roles on the cell viability, metastasis and tumor growth of OSSC cells. Additionally, EMT was also inhibited. Interestingly, upregulated let-7c-5p promoted apoptosis. Consequently, the abnormal expression of IGF2BP1 reversed functions of up-regulated let-7c-5p in OSCC cells.

Conclusion: These findings suggested that let-7c-5p behaves as an inhibitor regulating OSCC cell proliferation and migratory ability, which contributes to treatments by targeting IGF2BP1 in patients suffered from OSCC.

Keywords: Let-7c-5p, IGF2BP1, OSCC, Suppressor.